GenomeCraft is a contract research organization that offers a range of computational metabolic engineering services. GenomeCraft specializes in genome scale modeling for the identification of engineering targets. GenomeCraft was founded in 2017 by Igor Libourel. All computational tasks are performed in-house, and GenomeCraft partners with molecular biology and fermentation providers to perform wet lab tasks.
Publications by Igor Libourel:
Krumholz EW, Libourel IGL (2017) Thermodynamic Constraints Improve Metabolic Networks. Biophysical Journal 113 (3), 679-689Bowden SD, Palani NP and Libourel IGL (2016) Stringent Control of FLP Recombinase in E. coli. Journal of Microbiological Methods 133: 52-54
Goldford JE, Libourel IGL (2016) Unsupervised Identification of Isotope-Labeled Peptides. Analytical Chemistry, 2016, 88 (11), 6092-6099
Krumholz EW, Libourel IGL (2015) Sequence-based network completion reveals the integrality of missing reactions in metabolic networks. The Journal of Biological Chemistry 290: 19197-19207
Allen DK, Goldford J, Gierse JK, Mandy D, Diepenbrock C, Libourel IGL (2014) Quantification of Peptide m/z Distributions from C-13-Labeled Cultures with High-Resolution Mass Spectrometry. Analytical Chemistry 86: 1894-1901.
Allen DK, Evans BS, Libourel IGL (2014) Analysis of Isotopic Labeling in Peptide Fragments by Tandem Mass Spectrometry. PLoS ONE 91537
Burdge DA, Libourel IGL (2014) Open Source Software to Control Bioflo Bioreactors. PLoS ONE 92108
Mandy DE, Goldford JE, Yang H, Allen DK, Libourel IGL (2014) Metabolic Flux Analysis using C-13 Peptide Label Measurements. Plant Journal 77: 476-486
Yang H, Krumholz EW, Brutinel ED, Palani N, Sadowsky MJ, Odlyzko AM, Gralnick JA, Libourel IGL (2014) Genome-Scale Metabolic Network Validation of Shewanella oneidensis using Transposon Insertion Frequency Analysis. PLoS Comput Biol 10(9): e1003848
Krumholz EW, Yang H, Weisenhorn P, Henry CS, Libourel IGL (2012) Genome-wide Metabolic Network Reconstruction of the Picoalga Ostreococcus. Journal of Experimental Botany 63: 2353-2362
Allen DK, Libourel IGL, Shachar-Hill Y (2009) Metabolic Flux Analysis in Plants: Coping with Complexity. Plant Cell and Environment 32: 1241-1257
Libourel IGL, Shachar-Hill Y (2008) Metabolic Flux Analysis in Plants: From Intelligent Design to Rational Engineering. Annu al Review of Plant Biology 59: 625-650
Bethke PC, Libourel IGL, Aoyama N, Chung YY, Still DW, Jones RL (2007) The Arabidopsis Aleurone Layer Responds to Nitric Oxide, Gibberellin, and Abscisic Acid and is Sufficient and Necessary for Seed Dormancy. Plant Physiology 143: 1173-1188
Libourel IGL, Gehan JP, Shachar-Hill Y (2007) Design of Substrate Label for Steady State Flux Measurements in Plant Systems Using the Metabolic Network of Brassica napus embryos. Phytochemistry 68: 2211-1221
Bethke PC, Libourel IGL, Jones RL (2006) Nitric Oxide Reduces Seed Dormancy in Arabidopsis. Journal of Experimental Botany 57: 517-526
Bethke PC, Libourel IGL, Reinohl V, Jones RL (2006) Sodium Nitroprusside, Cyanide, Nitrite, and Nitrate Break Arabidopsis Seed Dormancy in a Nitric Oxide-Dependent Manner. Planta 223: 805-812
Libourel IGL, van Bodegom PM, Fricker MD, Ratcliffe RG (2006) Nitrite Reduces Cytoplasmic Acidosis under Anoxia. Plant Physiology 142: 1710-1717
Libourel IGL, Bethke PC, De Michele R, Jones RL (2006) Nitric Oxide Gas Stimulates Germination of Dormant Arabidopsis seeds: Use of a Flow-Through Apparatus for Delivery of Nitric Oxide. Planta 223: 813-820
Stoimenova M, Libourel IGL, Ratcliffe RG, Kaiser WM (2003) The Role of Nitrate Reduction in the Anoxic Metabolism of Roots – II. Anoxic Metabolism of Tobacco Roots with or Without Nitrate Reductase Activity. Plant and Soil 253: 155-167
Book Chapters:
Mori H, Takeuchi R, Otsuka Y, Bowden S, YokoYama K, Muto A, Libourel IGL, Wanner BL (2015). Toward Network Biology in E. coli. In: Babu M, editors. Prokaryotic Systems Biology.
Yang H, Mandy DE, Libourel IGL (2014). Optimal Design of Isotope Labeling Experiments. In: Methods in Molecular Biology, Sriram G, editors. Plant Metabolism: Methods and Protocols. Humana Press. 133-147
Bethke PC, Libourel IGL, Vitecek J, Jones RL (2011). Nitric Oxide Methods in Seed Biology. In: Methods in Molecular Biology, Kermode AR, editors. Seed Dormancy. Humana Press. 385-400
Bethke PC, Libourel IGL, Jones RL (2007). Nitric oxide in seed dormancy and germination Annual Plant Reviews Volume 27: Seed Development, Dormancy and Germination